Signalling

Part:BBa_K750102

Designed by: Sifan Wang,Yizhen Yan,Jianzhao Chi,Qingshu Wu   Group: iGEM12_XMU-China   (2012-09-21)

PtetcIT:atc sensor(J23119 promoter) with cI system

This part contains a constitutive promoter(BBa_J23119) and a tetR QPI(BBa_Q0040). It takes (anhydro)tetracyclin as the input signal, and the parts behind will produce corresponding output signal. In our project, this part is ligated with the CI protein expression system to make up a atc sensor with CI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
controlR0040
device_typesignalling
n/aPtetcIT:atc sensor(J23119 promoter) with cI system
proteinCI